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csimaging:csi [2013/09/10 09:57]
127.0.0.1 external edit
csimaging:csi [2013/09/10 14:37] (current)
lpolerec
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 The value of the method was demonstrated by imaging internal pH in the cytoplasm and vacuoles of the sulfur oxidizing bacteria //​Beggiatoa//​ sp. (Figure 1). This was considered as a method to evaluate predictions of genomic analyses, but appeared not possible with existing techniques. The RCSI method can be used for other compounds too, such as Ca2+ and Mg2+ concentration in living cells, while in sediments. The value of the method was demonstrated by imaging internal pH in the cytoplasm and vacuoles of the sulfur oxidizing bacteria //​Beggiatoa//​ sp. (Figure 1). This was considered as a method to evaluate predictions of genomic analyses, but appeared not possible with existing techniques. The RCSI method can be used for other compounds too, such as Ca2+ and Mg2+ concentration in living cells, while in sediments.
  
-{{ :​beggiatoa-ph.png?​400 }}+{{ :imaging:​beggiatoa-ph.png?​400 }}
 **Figure 1:** Transmission (gray) and pH (color-coded) images of a //​Beggiatoa//​ filament. The vacuole is acidic (pH2), the cytoplasm is neutral (pH7-8). The ∆pH across the vacuole membrane was dissipated by nigericin, a Na+-H+ exchanger. **Figure 1:** Transmission (gray) and pH (color-coded) images of a //​Beggiatoa//​ filament. The vacuole is acidic (pH2), the cytoplasm is neutral (pH7-8). The ∆pH across the vacuole membrane was dissipated by nigericin, a Na+-H+ exchanger.
  
csimaging/csi.txt · Last modified: 2013/09/10 14:37 by lpolerec