Matlab scripts and functions that are now part of the O2dyn suite have been developed in 2002-2005 to facilitate various types of analysis of oxygen images obtained by the MOLLI system. The progress of the developments has not been kept since ~2005, since it was too time-consuming. Nevertheless, examples of what the programs can do can be seen in these two old manuals: o2dyn-index.pdf and o2dyn-devel.pdf.
The capabilities are also demonstrated in the publications listed here.
You can download the program from here. Note that you need Matlab to be able to run the program.